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rpoB [2019-06-07 17:17:13]
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rpoB [2019-06-07 17:17:13]

RNA polymerase beta subunit
Locus
BSU01070
Isoelectric point
4.73
Molecular weight
133.38 kDa
Protein length
1193 aa Sequence Blast
Gene length
3582 bp Sequence Blast
Function
transcription
Product
RNA polymerase beta subunit
Essential
yes
E.C.
2.7.7.6
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → Transcription → RNA polymerase
  • Groups of genes → Essential genes
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    121,919 → 125,500

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)

    • Protein family

  • RNA polymerase beta chain family (single member, according to UniProt)

    • Modification

  • phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 PubMed

    • Structure

  • 3IYD (RNA polymerase from E. coli, 59% identity) PubMed
  • 3MLQ (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed

    • Localization

  • membrane associated PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

    • Expression and Regulation

    Operons

    Genes
    rpoB-rpoC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, in stringent response

    • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the leader mRNA is processed upstream of rpoB by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    LacZ fusion

  • pGP2683 (cat) based on pAC5, available in Jörg Stülke's lab
  • pGP2682 (aphA3) based on pAC7, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

    • FLAG-tag construct

  • GP2043 rpoB-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab

    • References

    Reviews

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    Original publications

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